SPITALUL CLINIC COLENTINA
Pathological Anatomy Service - PATHUNIT

Equipment


Microscope Nikon Eclipse 100

Optical system: CFI optical system;
Illumination: 6V-20W halogen lamp, FL (field lens) unit;
Coarse and fine focusing: Coaxial coarse/fine focusing with cross roller guide incorporated;
Coarse motion: 37.7mm/rotation, Fine motion: 0.2mm/rotation;
Eyepiece tube: E2-TB binocular tube (inclination: 30°, 360° rotatable Siedentopf-type, interpupillary distance: 47–75mm);
Eyepiece lens: E1-CFI 10x (F.O.V.: 18mm);
Condenser: Abbe condenser, NA 1.25 (aperture diaphragm with position guide marking for 4x, 10x, 40x, 60x and 100x objectives);
33mm-diameter filters attachable (including standard blue filter); adjustable vertical condenser movement range: 10mm;
Dedicated objectives: The new CFI BE Plan objectives, which feature superior image flatness; chromatic aberration and field curvature are corrected over the entire field of view.CFI BE Plan Achromat 4x (NA 0.1/W.D. 25mm), 10x (NA 0.25/W.D. 6.7mm), 40x (NA 0.65/W.D. 0.6mm), 100x (NA 1.25/W.D. 0.14mm);
Stage: Double-plate rectangular mechanical stage, stage size: 155 (W) x 134 (D)mm, removal slide holder, travel area: 76 (W) x 40 (D)mm, vernier provided.

Microscope Eclipse 50i with multiple teaching heads

Optical system: CFI60 Infinity Optical System;
Coarse/fine focusing: Fine: 0.1mm per rotation, Coarse: 13.8mm per rotation, Refocusing function (with stopper);
Illumination: 6V-30W halogen lamp;
Built-in filter: ND8;
Eyepiece tube: Trinocular tube (for F.O.V. 22mm/25mm, observation/photo: 100/0, 0/100); Ergonomic binocular tube (for F.O.V. 22mm, inclination:10-30°, extension: 40mm);
Eyepiece lens: 10X (F.O.V.: 22mm);
Nosepiece: Sextuple nosepiece;
Dedicated objectives: Plan Achromat objectives with chromatic aberration and field curvature are corrected over the entire field of view.CFI BE Plan Achromat 4x (NA 0.1/W.D. 25mm), 10x (NA 0.25/W.D. 6.7mm), 20x (NA 0.4/W.D. 3.7mm), 40x (NA 0.65/W.D. 0.6mm), 100x (NA 1.25/W.D. 0.14mm);
Stage: Rectangular 159mm X 243mm surface stage, 78mm X 54mm cross travel (x-y movement), 1-slide or 2-slide specimen holder available (option);
Accessories: Teaching heads for 10 persons, Simple polarizing, luminous pointer, object marker.

Deep freezer (chest model) DF9017 Chest -86ºC Deep freezer ULT

Features:
• storage capacity 492 liter;
• natural refrigerants and environmental friendly cooling system; low noise level (50dBa) WhisperFreeze Technology with quiet running fans and very low gas system pressures;
• sophisticated control panel with all information and warnings for stored samples;
• temperature Range:  -40ºC down to -86ºC  (factory setpoint -80ºC);
• capacity: 492 litres (5 insulated inner sublids).

Ultracentrifuge SIGMA 3K30

• high speed refrigerated table top centrifuge for centrifugal forces of up to and more than 60 000 x g;
• cooling system, pre-selection from -20°C to +40°C;
• speed range from 20 to 30 000 rpm., selectable in steps of 1 rpm;
• time pre-setting infinite or up to 9h 59min.

Techne TC-3000 Thermal Cycler

• high speed - 60 minutes for a typical 30 cycle program;
• peltier technology, the TC-3000 is capable of fast heating and cooling rates less than 2.0–3.0°C per second;
• a heated lid, PC control and networking and 80-program memory;
• temperature Range: 4°–99° C.

Macroscopic trimming cabinet

• structure made entirely of AISI 304 stainless steel, thickness 15/10, with smooth surface and rounded edges;
• 6/7 mm accident prevention stratified frontal glass with manual vertical slide to contain toxic fumes inside the hood (in compliance with the regulation in force). Run stop at 230 mm from work surface (descent), at 470 mm from work surface (ascent);
• white polyethylene plate for pieces reduction - 350x450x20 mm (W x D x H) - provided with N. 4 support feet 30 mm high;
• large aspirated central basin, dedicated to liquids collection, covered by a removable perforated top;
• double washing system consisting in: 1) Shower, provided with extensible flexible arm, to clean the whole working surface 2) Cleaning system inside the central basin, provided with tap;
• electrofan for fumes aspiration. The air is purified passing through prefilter and filter before discharge.

Cryostate Microm HM 525

• section thickness from 1 to 100΅m;
• trim section thicknesses from 5 to 500 ΅m;
• horizontal feed range of 28mm / Vertical cutting stroke of 60mm;
• temperature of the cryo chamber controlled down to -35°C;
• peltier down to -60ºC on 4 freezing stations (27 total freezing stations);
• extra wide (69.5mm) glass anti-roll plate for easy flatening and positioning of sections;
• automatic defrosting with immediate defrosting and interruption function;
• disposable blade holder.

Water Baths

• water bath for stretching sectioned tissue specimens, with dimensions of water container 200 x 60 mm;
• temperature adjustable to 80°C;
• anodized black surface provides contrast for easy evaluation of the sections;
• a wide heated rim permits temporary storage of slides for drying.

Magnetic stirrer MR Hei-Mix D:

• electronic speed control with digital display;
• speed range: 100 - 1,400 rpm;
• speed accuracy: ± 2 %;
• enhanced heavy-duty stirring magnet drives stirring bar with ease;
• top plate made of corrosion-resistant stainless steel (V2A);
• plate diameter: 145 mm.

Ph-meter

It offers waterproof protection in a compact casing. The included multi-parameter electrode has a built-in temperature sensor. The electrode also contains a mini amplifier to render your measurements impervious to noise and electrical interference. The large display shows readings in an extended range from -2.00 to 16.00 pH and simultaneously shows temperature from -5.0 to 105.0 °C or 23 to 221°F.

Vortex V1 Biosan

• ensures quick and efficient stirring due to the orbital vibrations;
• continuous or touch sensitive operation;
• manual speed controller;
• rubber suction pads that prevent walking and absorb vibration.

Shandon Slide and Block Cabinets

• all units are stackable to form an integrated system;
• steel construction;
• slide Safe contains 14 drawers with a total capacity of 6,000 microscope slides;
• sealed top and bottom to ensure dust-free storage;
• finished in solvent-proof paint;
• each Slide Safe unit measures 480 x 480 x 130 mm;
• each Block Bank unit contains 8 drawers with an approximate total capacity of 3,000 blocks and dimensions of 480 x 480 x 275 mm.

Shandon Carousel Cassette MicroWriter

• permanently mark tissue cassettes with easy to read black type;
• foil tape and a heated stylus to imprint characters that maintain legibility even through processing solutions;
• marks approximately 20,000 characters per roll of tape;
• printing can be inverted for reading in either direction;
• imprints letters, numbers, Roman numerals, and special characters on the cassettes in four font sizes;
• ability to print barcodes on cassettes;
• prints on all 45° flat-faced cassettes.

Microscopes Olympus BX41 with suplemmental teaching head

Optical system: UIS2 optical syste;
Focus: Vertical stage movement: 25mm stage stroke with coarse adjustment limit stop High-sensitivity fine focusing knob (adjustment gradations: 1΅m);
Illuminator: Built-in Kohler illumination for transmitted light, 6V30W halogen bulb (pre-centered);
Revolving nosepiece: quintuple nosepiece;
Observation tube: Widefield binocular, inclined 30° (F.N. 22);
Stage: Ceramic-coated coaxial stage with rotating mechanism;
Objectives: Plan Achromats 4x, 10x, 40x and 100x oil;
Multi-head viewing attachments for two persons with built-in pointer allows teachers to highlight areas of interest.

STP 420D Tissue Processor

• dual processing chambers; independent programming for either chamber;
• the larger one operates utilizing a patented rotational movement. This rotational movement allows fluid exchange of up to 7 times faster than traditional tidal agitation. The secondary chamber can be operated in conjunction with or independent of the main chamber;
• touch screen operation;
• 4-5L paraffin reservoirs with patented quick-melt feature using multi-faceted cylindrical columns within the reservoirs;
• paraffin regeneration;
• internal UPS system;
• total cassette capacity of 420: 360 cassette capacity in rotational chamber, 60 cassette capacity in secondary chamber.

Microm EC 350 Parraffin Embedding Center

The embedding center features programmable operating times including automatic start. It contains two components, a dispensing console and a cryo console.

The Dispensing Console has a 5-liter paraffin reservoir with heated dispenser nozzle, heated work surfaces, heated forceps holders and a cold spot. It includes separate heated compartments for molds and cassettes. All heated surfaces are independently controlled.

The Cryo Console features a large, non-stick surface for cooling paraffin embedded blocks. Adjustable temperature range:

1. Dispensing Console:
• paraffin storage tank, right and left chambers and hot zone: 50-70 oC;
• tweezer storage: 50-75 oC.
2. Cryo Console:
• 0 to -15 oC.

Microtome Microm HM 340 E

• specimen moves toward the knife;

• section thickness range from 0,5 to 100΅m;

• trim thickness range from 5 to 500΅m;

• operating panel can be used on either side of instrument;

• specimen retraction on return stroke.

 

Water Baths

• water bath for stretching sectioned tissue specimens, with dimensions of water container 200 x 60 mm;
• temperature adjustable to 80°C;
• anodized black surface provides contrast for easy evaluation of the sections;
• a wide heated rim permits temporary storage of slides for drying.

CRUMA portable fume hoods

• illumination;
• liquid retention tray (2 litre capacity);
• safety glass work surface;
• anemometer for continuous control of the air face velocity and inside the fume hood;
• alarm in case of fan failure;
• door open alarm;
• system to detect the saturation of the filter using colorometric reactive tubes;
• incorporation of a digital timer with audible and visual indications, warning every 60 hours of the need to check the saturation level of the filter;
• structure made from 2 mm steel plate with acid resistant polymerised Epoxy coating;
• side panels and front doors of transparent 8 mm thick polymethyl methacrylate, highly resistant to fire and acids;
• low noise level centrifugal fans (50 dB), placed after the filter to prevent corrosion;
• fully programmable electronic circuit that can be customised on request;
• nominal air flow (cu.metre/hour): 205;
• average face velocity (metre/second): 0.50;
• noise level: 48 dB.

Analytical  Balance

The RADWAG WAS Series Analytical, Laboratory Balance is an efficient electronic instrument which has many functions and applications that provide excellent value in accuracy and productivity. This Analytical Balance features:

• automatic internal calibration system by time interval, internal temperature change or on demand which constantly ensures balance  within calibration specifications, reducing errors;
• easy to use keybpoard arrangement and menu – makes training and operation simple;
• adjustable filter settings – can adjust balance response to differing environmental conditions for more controlled results;
• intelligent full graphic backlight display with load indicator, bar graphs, date and time, adjustable contrast, function icons, statistical graphs enables easy use of the many functions and applications available;
• PS2 keyboard entry available – allow easy input of large amounts of data;
• anti-shock /antiload protection – helps protect sensitive mechanism from accidental damage or overload.

Thermo Scientific Microm CTM6 Glass Coverslipper

The Microm CTM6 is a highly efficient automatic glass coverslipper, capable of coverslipping up to 400 slides per hour with continuous slide rack loading capabilities. The slide baskets are capable of containing either 19 or 30 slides each.
The unit accepts coverslips of 40, 50, 55 and 60mm in length and a variety of commercially available mounting media. The volume of mounting medium deposited on the slide can be regulated during operation.

Vapors are controlled through an integrated charcoal filter or through an existing laboratory fume hood.

Ph-meter

It offers waterproof protection in a compact casing. The included multi-parameter electrode has a built-in temperature sensor. The electrode also contains a mini amplifier to render your measurements impervious to noise and electrical interference. The large display shows readings in an extended range from -2.00 to 16.00 pH and simultaneously shows temperature from -5.0 to 105.0 °C or 23 to 221°F.