SPITALUL CLINIC COLENTINA
Proiect de cercetare PED 141

Despre Proiect


CONTRACT DE CERCETARE PED141/2017

 Titlu

Rolul microparticulelor in mecanismele patogenetice ale complicatiilor vasculare la pacientii cu mieloproliferari cronice

 Abstract

Pacientii cu neoplasme cronice mieloprolifeative (MPNs) prezinta in mod frecvent complicatii trombotice. Asociat cu numar crescut de plachete circulante, riscul trombotic este de asemenea asociat cu o functionare defectuoasa a plachetelor si existenta unui status trombofilic; studii recente au aratat de asemenea prezenta in fluxul sangvin a microparticulelor (MPs) de provenienta plachetara. Productia acestora implica formarea unor vezicule pornind de la membrana plachetara ca rezultat al unor modificari ale citoscheletului celular si al unor proprietati fosfolipidice membranare. Gradul de exprimare al acestor MPs este considerat a fi un marker pentru diagnosticul si prognosticul unor patologii de tip proliferativ.

In acest proiect ne propunem stabilirea unor corelatii intre existenta in cantitate crescuta a MPs la pacienti cu MPNs prezentand risc trombotic ridicat. Identificarea precoce a acestor pacienti este foarte importanta pentru deciziile de tratament citoreductiv.

In cursul implementarii proiectului vom utiliza un ansamblu de tehnici moleculare si de metode biofizice de studiu pentru a evalua activitatea plachetara si proprietatile membranare  ale acestora, prezenta si proprietatile MPs in sangele pacientilor cu MPNs. Aceste date experimentale vor fi corelate cu scorurile de risc trombotic asa cum sunt ele determinate prin procedurile hematologice standard.

Existenta unor astfel de corelatii furnizeaza un nou marker molecular pentru predictia riscului trombotic la pacientii cu MPNs.

 Parteneri

 -     -     Spitalul Clinic Colentina Bucuresti (CO, Director de proiect Dr. Viola M. Popov)

 -     -     Universitatea de Medicina si Farmacie Carol Davila Bucuresti (P1, Responsabil de proiect Conf. Dr Mihaela G. Moisescu)

 Finantare

Program PNIII, Program 2 - Cresterea competitivitatii economiei romanesti prin cercetare, dezvoltare si inovare, Proiect experimental-demonstrativ

Valoare totala: 600 000 lei

Durata contractului: 18 luni

 Diseminarea rezultatelor

   1.      Assessment of Changes of Membrane Properties and Platelets Function in Patients with Chronic Myeloid Leukaemia

59th ASH Annual Meeting, 9-12 decembrie 2017, Atlanta, USA

will be published online-only on the ASH abstracts site on December 8, 2017, and will become part of the permanent ASH and Blood abstracts archive

   2.      Analiza modificarilor fluiditatii si potentialului de membrana trombocitara la pacientii cu neoplazii mieloproliferative cronice

V. Popov, A. Vladareanu, B. Matei, C. Matei, M. Omer, M. Andreescu, O. Patrinoiu, F. Mihai, M.G. Moisescu, E. Kovacs, T. Savopol, H. Bumbea

A V-a Conferinta Nationala de Hemostaza si Tromboza (SRHT), 2-4 noiembrie 2017, Bucuresti, Romania

Prezentare orala,

http://srht.ro/conferinta-srht-nov-2017

    3.      The analyses of thrombotic risk in chronic myeloproliferative neoplasms patients

V.M. Popov, A. Trifa, M. Omer, M. Andreescu, F. Mihai, F. Raicu, S. Chioveanu, A. M. Grigoriu

10th International Congress on Myeloproliferative Neoplasms and Chronic Myeloid Leukemia, 2-3 noiembrie 2017, New York 2017

Poster, P104,

https://www.emedevents.com/c/medical-conferences-2017/10th-international-congress-on-myeloproliferative-neoplasms-and-chronic-myeloid-leukemia-diagnosis-management-and-new-clinical-developments-1

   4.      Evaluation of Platelet Membrane Fluidity in Assessment of Thrombosis Risk at Chronic Myeloproliferative Neoplasms Patients

V. M. Popov, M. B. Matei, C. O. Matei, M. G. Moisescu, M. Omer, M. Andreescu, F. Mihai, E. Kovacs, A. M. Vladareanu, H. Bumbea,T. Savopol

10th International Congress on Myeloproliferative Neoplasms and Chronic Myeloid Leukemia, 2-3 noiembrie 2017, New York 2017

Poster, P105,

https://www.emedevents.com/c/medical-conferences-2017/10th-international-congress-on-myeloproliferative-neoplasms-and-chronic-myeloid-leukemia-diagnosis-management-and-new-clinical-developments-1

    5.      Modification of platelet function in chronic lymphoid leukemia treated with Ibrutinib

V. M. Popov, M. G. Moisescu, E. Kovacs, M. M. Bogdan, C. O. Matei, A. M. Vladareanu, H. Bumbea,T. Savopol

Lymphoma & Myeloma International Congress, 26-28 octombrie 2017, New York, USA

Poster, P-011,

http://www.imedex.com/lymphoma-myeloma-conference/

    6.      Assessment of changes of membrane fluidity and platelet membrane potential in patients with chronic myeloproliferative neoplasms

V. Popov, H. Bumbea, B. Matei, C. Matei, M. Omer , F. Mihai , M. Moisescu, T. Savopol

VIIIth International Eurasian Hematology Oncology Congress, 18-21 octombrie 2017, Istanbul, Turkey

Prezentare orala, OP-19 Leukemia Research, October 2017, 61:Suppl 1, ISSN 0145-2126,

http://ehoc.org/2017/#

7.      Optical properties of electroporated cells as reveled by digital holographic microscopy

M. G. Moisescu, M. Mihailescu, V. L. Calin, E. Scarlat, E. Kovacs, A. V. Baluta, T. Savopol

2nd World Congress on Electroporation and Pulsed Electric Field, Medicine, Food and Environmental Technologies, 22-29 septembrie 2017, Norfolk, USA

Prezentare orala, OR-050,

https://wc2017.electroporation.net/event/program

    8.      Insights of electroporated cells as revealed by digital holographic microscopy

M. G. Moisescu, M. Mihailescu, V. L. Calin, A. V. Baluta, E. Scarlat, E. Kovacs, T. Savopol

19th IUPAB Congress and 11th EBSA Congress, 16-20 iulie 2017, Edinburg, Marea Britanie

Poster, P686:B37, European Biophysics Journal with Biophysics Letters, July 2017, 46:Suppl 1, p S294,

http://www.iupab2017.org/IOP/media/uploaded/EVIOP/event_607/Poster_Prog_Final.pdf

9.   Evaluation of cell malignancy using Digital Holographic Microscopy

V. L. Calin, M. Mihailescu, E. Scarlat, A. V. Baluta, E. Kovacs, T. Savopol, M. G. Moisescu

19th IUPAB Congress and 11th EBSA Congress, 16-20 iulie 2017, Edinburg, Marea Britanie

Poster, P674:B26, European Biophysics Journal with Biophysics Letters, July 2017, 46:Suppl 1, p S291,

http://www.iupab2017.org/IOP/media/uploaded/EVIOP/event_607/Poster_Prog_Final.pdf

   9.      The importance of platelet membrane fluidity and oxidative stress in thrombotic complications acquired by chronic myeloproliferative neoplasm patients

V. M. Popov, M. Andreescu, M. Omer, A. Trifa, F. Mihai, C. Dragan, O. Patrinoiu, M. G. Moisescu, T. Savopol, E. Kovacs, H. Bumbea, A. M. Vladareanu

22th EHA Congress, 22-25 iunie 2017, Madrid, Spania

Eposter, E1602 Hematologica, p 663

https://learningcenter.ehaweb.org/eha/2017/22nd/181378/viola.maria.popov.the.importance.of.platelet.membrane.fluidity.and.oxidative.html?f=topic=1574*media=2

    10.  International Summer School and Joint Symposium of Flow Cytometry, 6-9 iunie 2017, Bucuresti, Romania

Participare fara prezentari a Moisescu Mihaela si Miclea Luminita

https://sites.google.com/view/flowcytometry01/home